 Nucl:Cyto Segmentation in HCS Imaging Enabled by Far-Red Dye
25 Jun 2008 Scientific presentations at the recent HCA Europe conference in Prague confirm the nucleic acid-binding dye DRAQ5™ as counterstain of choice for cell-based imaging HCS assays. DRAQ5™ rapidly enters live or fixed cells to intensity discriminate between nuclear and cytoplasmic compartments. The resulting differential is ideal for compartment segmentation by automated image analysis tools. This offers new opportunities for cell-based assays where cell location, cell perimeter, cell shape and cell spread parameters can be used to define the assay at the single cell. DRAQ5™ permits the reliable tracking of fluorophore-tagged protein translocations and expression changes in GPCRs, kinases and other therapeutic targets in drug discovery and in vitro toxicology. The far-red, nucleic acid binding fluorophore DRAQ5™ avoids emission overlap wit...
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