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Adhesive Micropatterns for HCS: a Case Study with Blebbistatin
29 Jun 2010: This poster demonstrates that drug effects can be detected and measured in the submicromolar range, when they are barely observable on regular cell culture supports. In addition, a significant difference between treated and untreated cells is detected with only 20 analyzed cells.
CYTOO Inc.
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Microplate-Based Endotoxin Testing in GLP/GMP Environments with the VersaMax™ Absorbance Microplate Reader and Pyrochrome® Assay
07 Jun 2010: This application note describes a typical set-up for a standard curve in the Pyrochrome® Assay. The Pyrochrome® Assay together with the VersaMax Microplate Reader offers a sensitive and specific method for detecting endotoxin contamination in the production process of the pharmaceutical and medical device industries.
Molecular Devices
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LpxC RapidFire® Screening Analysis
03 Jun 2010: LpxC represents an attractive target for therapeutics. This application note presents a RapidFire high‐throughput mass spec method for both the gram positive and the gram negative bacteria LpxC enzyme. This method screens the LpxC catalyzed reaction using the native reaction substrate, resulting in significant number of hits for client lead discovery programs.
BIOCIUS Life Sciences
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High-Throughput Low-Volume DNA Quantification and Analysis
03 Jun 2010: This application note details the very high speed at which the Thermo Scientific Multiskan GO can perform full DNA quantification and spectral analysis on large numbers of low volume (5 - 10 µl) samples. With the Multiskan® GO, measurement of full DNA spectra between 220-380 nm from 96 samples can be performed in less than three minutes, providing a spectral measuring capacity of over 2,000 samples per hour.
Thermo Fisher Scientific
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Are Natural Products Delivering? Experiences and Recommendations from 27 Screening Campaigns
04 May 2010: In this application note from BioFocus, microbial strain subfractions are generated and used in high-throughput screens (HTS) for analysis with regard to diversity, selectivity and strain origin. Natural compounds were identified during the follow up of subfraction screening programs and their chemical properties have been analyzed. Searches in natural product databases revealed that 135 of the 645 identified natural compounds are novel compounds.
BioFocus
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Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
21 Apr 2010: In this application note the POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in the UV range. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid dynamics.
BMG LABTECH
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Simplifying Peptide Bioanalysis
08 Apr 2010: This application note demonstrates that the Oasis PST μElution Method Development plate and protocol can greatly simplify extraction method development and rapidly identify an appropriate SPE sorbent and selective starting protocol for peptide bioanalysis. Such standardized approaches for determining optimal SPE recovery and concentration, and MRM-based LC/MS analysis may help to shorten time to market for peptide drugs.
Waters
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RapidFire® In Vitro ADME Applications
08 Apr 2010: This application note demonstrates the correlation between traditional LC-MS and RapidFire for a spectrum of ADME assays. The RapidFire® MS System is shown to perform in vitro ADME assays rapidly and without modifications to existing assay methods. Sample cycle times of approximately 6 seconds can also be achieved obtaining a 7 point IC50 curve in 42 seconds.
BIOCIUS Life Sciences
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Automation of Multiplexed Cell-Based Assay to Measure Induction of Gene Expression and Activity of Cytochrome P450 Isoform 3A4
08 Apr 2010: This application note demonstrates the automation of a cell-based multiplexed assay to explore how a drug can induce gene expression and inhibit activity of the same isoform, creating a useful tool to measure the stimulatory and inhibitory effects of a drug. Activity and induction of gene expression of CYP3A4 were measured using a luminogenic, cell-membrane permeable 3A4-specific substrate and a luciferin based reporter gene assay.
BioTek Instruments, Inc.
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Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH’s NEPHELOstar
08 Apr 2010: Laser nephelometry has been shown to be a reliable technique for the measurement of drug solubility in 96-well plate format. In this application note the effects of complexation on drug antimycotic activity is described. Laser nephelometry can distinguish between the concentration at which the drug just goes into or just comes out of solution. This is presented in phase solubility studies.
BMG LABTECH
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adme-toxicology
BioTek’s Synergy H4™ Hybrid Reader is LanthaScreen® Plus Certified 
